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rabbit anti collagen iv antibody  (Bio-Rad)


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    Structured Review

    Bio-Rad rabbit anti collagen iv antibody
    Rabbit Anti Collagen Iv Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+collagen+iv/Rabbit+anti+Mouse+Collagen+IV/pmc13128929-71-37-42
    Average 95 stars, based on 249 article reviews
    rabbit anti collagen iv antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Non-Invasive Functional Gene Delivery to the Central and Peripheral Nervous System Across Species
    Article Snippet: .. E. Immunohistochemistry and imaging In the AAV characterization experiment in mice, tissue sections, typically 100-um thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti- podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100B from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker.

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-0300, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat#07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Incubation:

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Non-Invasive Functional Gene Delivery to the Central and Peripheral Nervous System Across Species
    Article Snippet: .. E. Immunohistochemistry and imaging In the AAV characterization experiment in mice, tissue sections, typically 100-um thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti- podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100B from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker.

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-0300, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat#07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Blocking Assay:

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Non-Invasive Functional Gene Delivery to the Central and Peripheral Nervous System Across Species
    Article Snippet: .. E. Immunohistochemistry and imaging In the AAV characterization experiment in mice, tissue sections, typically 100-um thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti- podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100B from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker.

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-0300, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat#07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Data-independent acquisition:

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Non-Invasive Functional Gene Delivery to the Central and Peripheral Nervous System Across Species
    Article Snippet: .. E. Immunohistochemistry and imaging In the AAV characterization experiment in mice, tissue sections, typically 100-um thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti- podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100B from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. .. In the AAV characterization experiment in mice, tissue sections, typically 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abeam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100β from Abeam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abeam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker.

    Article Title: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Article Snippet: .. In the AAV characterization experiment in mice, tissue sections, 100-μm thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-0300, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat#07-1401, diluted: 1:500; Anti-S100β from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Immunofluorescence:

    Article Title: A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy.
    Article Snippet: .. Antibodies used for immunofluorescence staining: anti-CD31 (Dako, Mouse, Cat# M0823, RRID AB_2114471), anti-CD11b (Sigma-Aldrich, Rabbit, Cat# SAB5600105, RRID:AB_2910138), anti-Collagen IV (BioRad, Rabbit, Cat# 2150-0140, RRID:AB_2082644), anti-Hif1α (Bethyl (Thermofisher), Rabbit, Cat# A300-286A, RRID:AB_2117114), CleavedCaspase 3 (Cell Signaling, Cat# 9661, RRID:AB_2341188). .. Secondary antibodies: goat anti-mouse-Cy5 (Jackson ImmunoResearch, Cat# 115-175-166, RRID:AB_2338714), goat anti-rabbit-Alexa Fluor 750 (Thermofisher, Cat# A-21039, RRID:AB_2535710).

    Article Title: A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy
    Article Snippet: .. Antibodies used for immunofluorescence staining: anti-CD31 (Dako, Mouse, Cat# M0823, RRID AB_2114471), anti-CD11b (Sigma-Aldrich, Rabbit, Cat# SAB5600105, RRID:AB_2910138), anti-Collagen IV (Bio-Rad, Rabbit, Cat# 2150-0140, RRID:AB_2082644), anti-Hif1α (Bethyl (Thermofisher), Rabbit, Cat# A300-286A, RRID:AB_2117114), Cleaved-Caspase 3 (Cell Signaling, Cat# 9661, RRID:AB_2341188). .. Secondary antibodies: goat anti-mouse-Cy5 (Jackson ImmunoResearch, Cat# 115-175-166, RRID:AB_2338714), goat anti-rabbit-Alexa Fluor 750 (Thermofisher, Cat# A-21039, RRID:AB_2535710).

    Staining:

    Article Title: A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy.
    Article Snippet: .. Antibodies used for immunofluorescence staining: anti-CD31 (Dako, Mouse, Cat# M0823, RRID AB_2114471), anti-CD11b (Sigma-Aldrich, Rabbit, Cat# SAB5600105, RRID:AB_2910138), anti-Collagen IV (BioRad, Rabbit, Cat# 2150-0140, RRID:AB_2082644), anti-Hif1α (Bethyl (Thermofisher), Rabbit, Cat# A300-286A, RRID:AB_2117114), CleavedCaspase 3 (Cell Signaling, Cat# 9661, RRID:AB_2341188). .. Secondary antibodies: goat anti-mouse-Cy5 (Jackson ImmunoResearch, Cat# 115-175-166, RRID:AB_2338714), goat anti-rabbit-Alexa Fluor 750 (Thermofisher, Cat# A-21039, RRID:AB_2535710).

    Article Title: A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy
    Article Snippet: .. Antibodies used for immunofluorescence staining: anti-CD31 (Dako, Mouse, Cat# M0823, RRID AB_2114471), anti-CD11b (Sigma-Aldrich, Rabbit, Cat# SAB5600105, RRID:AB_2910138), anti-Collagen IV (Bio-Rad, Rabbit, Cat# 2150-0140, RRID:AB_2082644), anti-Hif1α (Bethyl (Thermofisher), Rabbit, Cat# A300-286A, RRID:AB_2117114), Cleaved-Caspase 3 (Cell Signaling, Cat# 9661, RRID:AB_2341188). .. Secondary antibodies: goat anti-mouse-Cy5 (Jackson ImmunoResearch, Cat# 115-175-166, RRID:AB_2338714), goat anti-rabbit-Alexa Fluor 750 (Thermofisher, Cat# A-21039, RRID:AB_2535710).

    Immunohistochemistry:

    Article Title: Non-Invasive Functional Gene Delivery to the Central and Peripheral Nervous System Across Species
    Article Snippet: .. E. Immunohistochemistry and imaging In the AAV characterization experiment in mice, tissue sections, typically 100-um thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti- podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100B from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    Imaging:

    Article Title: Non-Invasive Functional Gene Delivery to the Central and Peripheral Nervous System Across Species
    Article Snippet: .. E. Immunohistochemistry and imaging In the AAV characterization experiment in mice, tissue sections, typically 100-um thick, were first incubated in the blocking buffer (10% normal donkey serum (NDS), 0.1% Triton X-100, and 0.01% sodium azide in 0.1 M PBS, pH 7.4) with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti- podocalyxin from R&D Systems Cat # AF1556, diluted 1:100; Anti-CD13 from AbD Serotec, Cat # MCA2183EL, diluted 1:100; Anti-CNN-1 (Calponin 1) from Abcam, Cat # Ab46794, diluted 1:100; Anti-Collagen-IV, from AbD, Serotec Cat # 2150-1470, diluted 1:300; Anti-GFAP from Invitrogen, Cat # 13-030, diluted 1:600; Anti-HA from Roche, Cat# 11867423001, diluted 1:200; Anti-GFP from Aves Labs, Cat# GFP-1020, diluted 1:500; Anti-GLUT1 from Millipore, Cat# 07-1401, diluted: 1:500; Anti-S100B from Abcam, Cat# ab52642, diluted: 1:500; Anti-NeuN from Abcam, Cat# ab177487, diluted: 1:500) for 24 h at room temperature (RT) on a rocker. ..

    other:

    Article Title: Dynamics of compartment-specific proteomic landscapes of hepatotoxic and cholestatic models of liver fibrosis
    Article Snippet: Antibody , Anti-collagen IV (Rabbit polyclonal) , Bio-Rad , 2150-1470 , IHCP (1:250).

    Article Title: A humanized breast cancer microorganoid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy
    Article Snippet: The cells were seeded at 2000 tumor cells/well with the respective broblast numbers in 200 μL broblast growth media (FGM-2, Lonza, Switzerland).



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